What’s Our Antigen Rapid Test Kit
This antigen rapid test kit is a lateral flow immunoassay intended for the qualitative detection of COVID-19 antigen in direct nasopharyngeal or oropharyngeal swabs from suspected individuals with virus infection by their healthcare provider, which can be used as direct evidence of viral infection.
[Components & Advantages]
√Quick test results in 15 minutes
√Easy to collect samples
√Colloidal gold used
Other Types of COVID-19 Test Kits
There are two kinds of Tests Available for COVID-19: viral tests and antibody tests. Nucleic acid test (PCR test) and antigen rapid test are both viral tests. A viral test tells you if you have a current infection. An antibody test tells you if you had a past infection.
How to Choose the Right Coronavirus Test
Please find the answer in the picture below:
Using a nasal or throat swab to get a fluid sample, this antigen rapid test kit can get results in 15 minutes.
For the T line, colloidal gold against COVID-19 antigen is used as a detector and coated on the conjugate pad. For the C line, colloidal gold conjugated antibody from the T line is used as the detector and goat anti-mouse IgG antibody is used as the capture and coated on nitrocellulose membrane.
When the COVID-19 antigen in the sample is combined with the colloidal gold-labeled protein antibody to form an antigen-antibody gold complex and fixed on the nitrocellulose membrane, a red band will appear on the T line.
1. Read the instruction carefully before using the test kit and return it to room temperature.
2. Take out the test card from the foil bag, put it on the level table and make a mark;
3. Add 80μl sample from the sample buffer into the sample well of the card with pipette, starting the timer;
4. Wait for 1-20 minutes until the test line appear red and record the result as a positive. If the test line does not appear red within 20 minutes, the record is negative.
[Interpretation of Results]
1. Positive: C line appears red, T line appears red, which indicates the COVID-19 antigen in the sample is detected.
2. Negative: C line appears red, T line does not appears red, which indicates the COVID-19 antigen in the sample is not detected.
3. Invalid: C line does not appear red.
1. When collecting the specimen of nasopharyngeal swabs or throat swabs, please follow the collection guidelines on the CDC website for additional information.
2. After collection, please remove the seal from the top of the buffer bottle containing the extraction buffer.
3. Place and soak the patient’s cotton swab in the extraction buffer for 10 seconds and then stir it evenly by rotating the swab against the side of the bottle 5 times.
4. Take out the patient swab while squeezing the middle of the extraction bottle to remove the liquid from the swab.
5. Discard the swab in the biohazard waste.
1. It was evaluated using COVID-19 vaccine samples (Tab 1). The vaccine sample was diluted with dilution buffer as 100, 1000, 10000, 100000 times and showed positive by 30000 times dilution.
2. It was evaluated using dilution buffer with the addition of 10, 1, 0.1, 0.01 and 0.001ng/ml of N protein of coronavirus antigen in our lab(Tab 2). The positive response of the coronavirus antigen test system showed 100% sensitivity by 0.01ng/ml.
Testing Specificity was evaluated using specimen collection of nasal swabs collected from healthy subjects in our lab (Tab 3). The negative agreement of the COVID-19 antigen test system showed 99% specificity for the COVID-19 antigen in 100 blood samples collected from healthy Subjects.
We have the stability and lifetime inspection going on for one month and did not find any significantly change of testing effects.
[Storage & Validity]
This kit shall be stored at 4℃-30℃, with a validity period of 12 months. After the aluminum foil bag is opened, it should be used within 15 minutes.
1. The test performance depends on the number of virus (antigens) in the sample, and may relate to viral culture results performed on the same sample, or not.
2. Specimens should be tested as quickly as possible after specimens collection.
3. If the extraction buffer is not used enough, false negative result may occur.
4. If the antigen level in the sample is below the detection limit of the test, a negative test result may occur.
5. If a specimen is improper collected, transported or handled, false negative results may occur.
6. If swabs are stored in a paper sheath after specimen collection, it may appear false negative result.
7. Positive test results do not rule out co-infections with other pathogens.